Cell envelopes of chemotaxis mutants of Escherichia coli rotate their flagella counterclockwise

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Cell envelopes of chemotaxis mutants of Escherichia coli rotate their flagella counterclockwise.

Flagella rotated exclusively counterclockwise in Escherichia coli cell envelopes prepared from wild-type cells, whose flagella rotated both clockwise and counterclockwise, from mutants rotating their flagella counterclockwise only, and even from mutants rotating their flagella primarily clockwise. Some factor needed for clockwise flagellar rotation appeared to be missing or defective in the cel...

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Fumarate or a fumarate metabolite restores switching ability to rotating flagella of bacterial envelopes.

Flagella of cytoplasm-free envelopes of Escherichia coli or Salmonella typhimurium can rotate in either the counterclockwise or clockwise direction, but they never switch from one direction of rotation to another. Exogenous fumarate, in the intracellular presence of the chemotaxis protein CheY, restored switching ability to envelopes, with a concomitant increase in clockwise rotation. An increa...

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Characterization of Escherichia coli chemotaxis receptor mutants with null phenotypes.

Hydroxylamine mutagenesis was used to alter the tar gene that encodes the transmembrane Tar protein required for chemotaxis. Mutants defective in chemotaxis were selected, and the mutation was characterized by DNA sequencing. Two classes of mutations were found: nonsense and missense. The nonsense mutations were distributed throughout the gene, while the missense mutations were found to cluster...

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Flagella of Escherichia coli spheroplasts.

Recently, Z. Vaituzis and R. N. Doetsch (J. Bacteriol. 89:1586, 1965) described the relationship between flagellation and motility in penicillin-induced spheroplasts of Salmonella typhimurium, and they concluded that the formation of flagella was dependent upon prior cell wall synthesis. This conclusion has now been confirmed with Escherichia coli (ATCC 13070), the results found being identical...

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Expression of tolC and organic solvent tolerance of Escherichia coli ciprofloxacin resistant mutants

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ژورنال

عنوان ژورنال: Journal of Bacteriology

سال: 1985

ISSN: 0021-9193,1098-5530

DOI: 10.1128/jb.162.1.451-453.1985